|Education||MS, Johns Hopkins University|
The molecular and cellular changes involving endodermal commitment and hepatic specification in humans is not clearly defined due to a lack of human-relevant model systems. Most of our knowledge of these processes stems from developmental or organogenesis studies heavily reliant on animal model systems like zebra fish and mice.
The advent of Induced Pluripotent Stem (iPS) Cell technology provides a relatively unexplored and potentially more appropriate model system to pursue human-relevant developmental studies. Particularly, the recent advances in directed differentiation of human iPS cells into multi-stage hepatic cells offer a relevant platform for dissecting the molecular events associated with cell fate changes occurring during endodermal commitment and future hepatic specification. Very little is known about the major cell fate regulators governing each stage of hepatic differentiation, such as definitive endoderm, hepatic progenitor and mature hepatocyte. My project deals with dissecting the molecular mechanisms underlying and governing cell fate determination and progression through endodermal and hepatic differentiation and maturation. This work will potentially enhance our understanding of human hepatogenesis, as well as liver pathogenesis.
Kurbanov F, Kim Y, Latanich R, Chaudhari P, El-Diwany R, Knabel M, Kandathil AJ, Cameron A, Cox A, Jang YY, Thomas DL, Balagopal A. IFNL3 genotype is associated with differential induction of IFNL3 in primary human hepatocytes. Antivir Ther. 2015;20(8):805-14. doi: 10.3851/IMP2974. Epub 2015 Jun 25. PMID: 26109548
Smith C, Abalde-Atristain L, He C, Brodsky BR, Braunstein EM, Chaudhari P, Jang YY, Cheng L, Ye Z. Efficient and allele-specific genome editing of disease loci in human iPSCs. Mol Ther. 2015 Mar;23(3):570-7. doi: 10.1038/mt.2014.226. Epub 2014 Nov 24. PMID: 25418680
Chaudhari P, Prasad N, Tian L, Jang YY. Determination of Functional Activity of Human iPSC-Derived Hepatocytes by Measurement of CYP Metabolism. Methods Mol Biol. 2016;1357:383-94. doi: 10.1007/7651_2014_145. PMID: 25410290
Chaudhari P, Ye Z, Jang YY. Roles of reactive oxygen species in the fate of stem cells. Antioxid Redox Signal. 2014 Apr 20;20(12):1881-90. doi: 10.1089/ars.2012.4963. Epub 2012 Nov 19. Review. PMID: 23066813