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Elisabeth Heuston

Class 2006
Education BA, Dartmouth
Email
Mentor

Dr. Robert Arceci

Current Position Research Fellow
Location

National Human Genome Research Institute, (NHGRI)

Research Interests

The role of PASG in genomic methylation

The proliferation-associated SNF2-like gene, PASG, is a member of the chromatin remodeling family, involved in genomic methylation. Previous studies have shown that a high percentage of AML and ALL patients have altered PASG splicing, causing an in-frame deletion of an evolutionarily conserved functional domain. Cells lacking PASG show genomic hypomethylation, genomic instability and a replicative senescence phenotype. PASG deficient mouse embryo fibroblasts, from PASG knockout mice, can on occasion circumvent replicative senescence spontaneously, or be immortalized by transfection with SV40 Large T antigen or exposure in culture to FGF. Of particular interest, FGF immortalized PASG -/- MEFs are more tumorigenic than PASG WT MEFs. The purpose of my research is to understand the functional role of PASG, and how expression of the splicing variant in mouse embryonic fibroblasts changes global genomic methylation patterns. This information will give us further understandin g about the biochemical activities and protein interactions required by PASG to maintain genomic methylation and stability as well as normal patterns of gene expression during development and leukemogenesis

Publications

Elmoneim AA, Heuston E, Wai DH, Triche T, Arceci RJ. Synergisitic and Antagonistic AML Cell Type-specific Responses to 5-Aza-2-deoxycitidine and 1-h-D-Arabinofuranoside. Anticancer Res. 2016 Feb;36(2):691-6.PMID:26851026

Heuston EF, Lemon KT, Arceci RJ. The Beginning of the Road for Non-Coding RNAs in Normal Hematopoiesis and Hematologic Malignancies. The Beginning of the Road for Non-Coding RNAs in Normal Hematopoiesis and Hematologic Malignancies. Front Genet. 2011 Dec 28;2:94. doi: 10.3389/fgene.2011.00094. eCollection 2011.PMID:22303388