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Sanjay K. Jain, MD

Department Affiliations Pediatrics
Rank Associate Professor
Office Phone 410-502-8241
Lab Phone 410-614-3051
Fax 410-614-8173
SOM Address 109 CRB II



Research Interests

My laboratory focuses on studying the pathogenesis of bacterial diseases, with a major focus on tuberculosis (TB). One of the unique features of my laboratory is the use of noninvasive molecular imaging technologies to noninvasively monitor disease in live animals. I direct a core molecular imaging facility – Center for Infection and Inflammation Imaging Research (Ci3R) –with capacity for PET, SPECT, CT and ultrasound imaging of infected animals ( The goals are to develop novel methods to better understand, diagnose, treat, and prevent infections in humans. For example, given that current imaging techniques for infections are non-specific, we have developed a systematic approach that exploits unique biochemical pathways in bacteria to develop novel pathogen-specific imaging tracers. We have screened hundreds of small molecules to develop a pipeline of bacteria-specific positron emission tomography (PET)-based imaging tracers capable of reliably and noninvasively differentiating infection from cancer and other non-infectious inflammatory process. We have also developed imaging techniques to noninvasively determine antimicrobial penetration into infected lesions, and understand lesions-specific host-responses. These data will help in the development of better antimicrobial treatments by optimizing drug-concentrations in infected tissues, and the use of host-directed therapies. We have developed or utilize several small animal models (mice, rabbits, etc.) – pulmonary and cavitary TB, TB meningitis, and several murine models for pyogenic bacteria. Finally, we also translate our preclinical findings to the clinic and are testing some novel imaging tracers developed in our laboratory in human studies.


  • Lentz CS, Ordonez AA, Kasperkiewicz P, Greca FL, O’Donoghue AJ, Schulze CJ, Powers JC, Craik CS, Drag M, Jain SK, Bogyo M. Design of selective substrates and activity-based probes for Hydrolase Important for Pathogenesis 1 (HIP1) from Mycobacterium tuberculosis. ACS Infect. Dis. 2016 (in press) doi: 10.1021/acsinfecdis.6b000922016.
  • Ordonez AA, Tasneen R, Pokkali S, Xu Z, Converse PJ, Klunk MH, Mollura DJ, Nuermberger EL, Jain SK. Mouse Model of Pulmonary Cavitary Tuberculosis and Expression of Matrix Metalloproteinase-9. Dis Model Mech. 2016; 9, 779-788 doi:10.1242/dmm.025643. PMID: 27482816
  • Salazar-Austin N, Ordonez AA, Hsu A, Benson JE, Mahesh M, Menachery E, Razeq JH, Salfinger M, Starke JR, Milstone AM, Parrish N, Nuermberger EL, Jain SK. Extensively Drug-Resistant Tuberculosis in a Young Child after Travel to India. Lancet Infect Dis 2015;15:1485-91. PMID: 26607130
  • DeMarco VP, Ordonez AA, Klunk M, Prideaux B, Wang H, Zhuo Z, Tonge PJ, Dannals RF, Holt DP, Lee CK, Weinstein EA, Dartois V, Dooley KE, Jain SK. Determination of 11C-Rifampin Pharmacokinetics within Mycobacterium tuberculosis-Infected Mice Using Dynamic Positron Emission Tomography Bioimaging. Antimicrob Agents Chemother. 2015 Sep;59(9):5768-74. PMID: 26169396
  • Ordonez AA, Pokkali S, DeMarco VP, Klunk M, Mease RC, Foss CA, Pomper MG, Jain SK. Radioiodo-DPA-713 Imaging Correlates with Bactericidal Activity of Tuberculosis Treatments in Mice. Antimicrob Agents Chemother. 2015 Jan;59(1):642-9. doi: 10.1128/AAC.04180-14. Epub 2014 Nov 17. PMID: 25403669
  • Weinstein EA, Ordonez AA, DeMarco VP, Murawski AM, Pokkali S, MacDonald EM, Klunk M, Mease RC, Pomper MG, Jain SK. Imaging Enterobacteriaceae infection in vivo with 18F-fluorodeoxysorbitol positron emission tomography. Sci Transl Med. 2014 Oct 22; 6(259):259ra146. doi: 10.1126/scitranslmed.3009815. [Cover article]. PMID: 25338757
  • Murawski AM, Gurbani S, Harper JS, Younes L, Jain SK*, Jedynak B. Imaging the Evolution of Reactivation Pulmonary Tuberculosis in Mice Using 18F-FDG PET. J Nucl Med. 2014 Oct;55(10):1726-9. doi: 10.2967/jnumed.114.144634. Epub 2014 Jul 31 *corresponding and co-last author. PMID: 25082854
  • Jain SK, Ordonez A, Gupte N, Kinikar A, Thakar M, Mave V, Jubulis J, Hatolkar S, Lalvani A, Kagal A, Gupta A, Bharadwaj R. Pediatric Tuberculosis in Young Children in India: A Prospective Study. Biomed Res Int. 2013:783698. doi: 10.1155/2013/783698. Epub 2013 Dec 10. PMCID: PMC3872373
  • Foss CA, Harper JS, Wang H, Pomper MG, Jain SK. Non-Invasive Molecular Imaging of Tuberculosis-Associated Inflammation with Radioiodinated DPA-713. J Infect Dis. 2013 Dec;208(12):2067-74. [Cover article]. PMCID: PMC3836460
  • Skerry C, Pokkali S, Pinn M, Be NA, Harper J, Karakousis PC, Jain SK. Vaccination with recombinant Mycobacterium tuberculosis PknD confers protection against bacterial dissemination to the brain in guinea pigs. PLoS One. 2013 Jun 11;8(6):e66310. doi: 10.1371/journal.pone.0066310. Print 2013. PMCID: PMC3679071
  • Skerry C, Harper J, Weir M, Bishai WR, Jain SK. Adjunctive TNF inhibition with standard treatment enhances bacterial clearance in a murine model of necrotic TB granulomas. PLoS One. 2012;7(6):e39680. doi: 10.1371/journal.pone.0039680. Epub 2012 Jun 27. PMCID: PMC3384606
  • Be NA, Bishai WR, Jain SK. Role of Mycobacterium tuberculosis pknD in the Pathogenesis of Central Nervous System Tuberculosis. BMC Microbiol. 2012 Jan 13;12:7. doi: 10.1186/1471-2180-12-7. Highly accessed. PMCID: PMC3322341
  • Harper J, Skerry C, Davis SL, Tasneen R, Weir W, Kramnik I, Bishai WR, Pomper MG, Nuermberger EL, Jain SK. Mouse model of necrotic TB granulomas develops hypoxic lesions. J Infect Dis. 2012 Feb;205(4):595-602. Epub 2011 Dec 23. PMCID: PMC3266133
  • Be NA, Klinkenberg LG, Bishai WR, Karakousis PK, Jain SK. Strain-dependent CNS dissemination in guinea pigs after Mycobacterium tuberculosis aerosol challenge. Tuberculosis (Edinb). 2011 Sep;91(5):386-9. PMCID: PMC3172348
  • Davis SL, Nuermberger EL, Um P, Vidal C, Jedynak B, Pomper MG, Bishai WR, Jain SK. Noninvasive pulmonary [18F]-2-fluoro-deoxy-D-glucose positron emission tomography correlates with bactericidal activity of tuberculosis drug treatment. Antimicrob Agents Chemother. 2009 Nov;53(11):4879-84. doi: 10.1128/AAC.00789-09. Epub 2009 Sep 8. PMCID: PMC2772305
  • Davis SL, Be NA, Lamichhane G, Nimmagadda S, Pomper MG, Bishai WR, Jain SK. Bacterial thymidine kinase as a non-invasive imaging reporter for Mycobacterium tuberculosis in live animals. PLoS One. 2009 Jul 16;4(7):e6297. doi: 10.1371/journal.pone.0006297. PMCID: PMC2706987
  • Be N, Lamichhane G, Grosset J, Tyagi S, Cheng Q, Kim KS, Bishai WR, Jain SK. Murine model to study Invasion and Survival of Mycobacterium tuberculosis in the Central Nervous System. J Infect Dis. 2008 Nov 15;198(10):1520-8 [Cover article]. PMID: 18956986