Cancer is a leading cause of death and morbidity in the United States and this problem is expected to grow as our life expectancy increases. We have focused on the genetics of cancer with a particular emphasis on colorectal cancer. We have previously identified genetic mutations responsible for the majority of inherited and sporadic forms of colorectal cancer. Future studies are aimed at further characterizing these mutated pathways using genetic manipulation of human cancer cells and powerful methods for gene expression analyses. In addition, we have major translational efforts intended improve the clinical management of cancer. These efforts include novel diagnostic and therapeutic approaches based on the knowledge of the genetic alterations that occur in cancer. A variety of novel approaches have been developed and employed to achieve the goals described above including, SAGE, Digital Karyotyping, Conversion, BEAMing and human somatic cell knockouts.
- Traverso, G., Shuber, A., Levin, B., Johnson, C., Olsson, L., Schoetz, D.J., Hamilton, S.R., Boynton, K., Kinzler, K.W., and Vogelstein, B. Detection of APC Mutations in Fecal DNA from Patients with Colorectal Tumors. New England Journal of Medicine 346: 311-320, 2002.
- Yan, H., Yuan, W., Velculescu, V.E., Vogelstein, B. and Kinzler, K.W. Allelic Variation in Human Gene Expression. Science 297: 1143, 2002.
- Dressman, D., Yan, H., Traverso, G., Kinzler, K.W. and Vogelstein, B. Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations. Proceedings of the National Academy of Sciences, U.S.A. 100: 8817-8822, 2003.
- Wang, Z., Vogelstein, B. and Kinzler, K.W. Phosphorylation of β-Catenin at S33, or T41 can occur in the absence of Phosphorylation at T45 in Colon Cancer Cells. Cancer Research 63: 5234-5235, 2003.
- Nanda, A., Carson-Walter, E.B., Seaman, S., Barber, T.D., Stampfl, J., Singh, S., Vogelstein, B., Kinzler, K.W., and St. Croix, B. TEM8 Interacts with the Cleaved C5 Domain of Collagen ά3 (VI). Cancer Research 64: 817-820, 2004.
- Samuels, Y., Wang, Z., Bardelli, A., Silliman, N., Ptak, J., Szabo, S., Yan, H., Gazdar, A., Powell, S.M., Riggins, G.J., Willson, J.K., Markowitz, S., Kinzler, K.W., Vogelstein, B. and Velculescu, V.E. High Frequency of Mutations of the PIK3CA Gene in Human Cancers. Science 304: 554, 2004.