Research in my laboratory is focused on understanding how neuronal connectivity is established during development. Our work investigates the function of extrinsic guidance cues and their receptors on axonal guidance, dendritic morphology, and synapse formation and function. For several years we have investigated how neural circuits are formed and maintained through the action of guidance cues, including but not limited to semaphorin proteins, their classical plexin and neuropilin receptors, and also novel receptors. We also employ a cross phylogenetic approach, using both invertebrate and vertebrate model systems, to understand how guidance cues regulate neuronal pathfinding, morphology, and synaptogenesis. Though broad in scope, our interrogation of the roles played by semaphorins, and also other guidance cues, provides insight into the regulation of neural circuit assembly and function. As a result of past and ongoing projects in my laboratory, I and my colleagues have extensive experience in mouse and fly genetic manipulations and a wide range of neuroanatomical analyses. Our current work includes a relatively new interest in understanding the origins of laminar organization in the central nervous system. This direction has resulted in our identification of guidance cues and receptors that regulate both specific and general aspects of neurite stratification in the mouse retina, in addition to the targeting of retinal ganglion cell axons to retinorecipient midbrain targets.
- Functional assembly of accessory optic system circuitry critical for compensatory eye movements. Sun LO, Brady CM, Cahill H, Al-Khindi T, Sakuta H, Dhande OS, Noda M, Huberman AD, Nathans J, Kolodkin AL. Neuron. 2015 May 20;86(4):971-84. Epub 2015 May 7.PMID:25959730
- Riccomagno, M.M., Sun, L.O., Brady, C.M., Alexandropoulos, K., Seo, S., Kurokawa, M., and Kolodkin, A.L. (2014). Cas adaptor proteins organize the retinal ganglion cell layer downstream of integrin signaling. Neuron, 81, 779-786 . [PMCID: PMC3988023].
- Tran, T.S., Rubio, M.E., Clem, R.L., Johnson, D.,Case, L.C., Tessier-Lavigne, M., Huganir, R.L., *Ginty, D.D., and *Kolodkin, A.L. (2009). Secreted semaphorins control spine distribution and morphogenesis in the postnatal CNS.. Nature, 462, 1065–1069 (*corresponding authors). [PMCID: PMC2842559].
- Matsuoka, R.L., Nguyen-Ba-Charvet, K.T., Parray, A., Badea, T.C., Chédotal, A., and Kolodkin, A.L. (2011). Transmembrane semaphorin signalling controls laminar stratification in the mammalian retina. Nature, 470, 259–263. [PMCID: PMC3063100].
- Wu, Z., Sweeney, L.B. Ayoob, J.C., Chak, K., Andreone, B.J., Ohyama,T. Kerr, R., Luo, L, Zlatic, M., and Kolodkin, A.L. (2011). A combinatorial semaphorin code instructs the initial steps of sensory circuit assembly in the Drosophila CNS. Neuron, 70, 281–298. [PMCID: PMC3095019].
- Riccomagno, M, Hurtado, A., Wang, H.-B., Macopson, J.G.J., Griner, E.M., Betz, A., Brose, N. Kazanietz, M.G., and Kolodkin, A.L. (2012). The RacGAP-Chimaerin selectively mediates stereotyped hippocampal axon pruning. Cell 149, 1594–1606 [PMCID: PMC3395473].
- Sun, L.O., Jiang, Z., Rivlin-Etzion, Michal, Hand, R., Brady, C., Matsuoka, R.L., Yau, K.-w., Feller, M.B., and Kolodkin, A.L. (2013). On versus Off direction selective retinal circuits require different molecular mechanisms for functional assembly (Article). Science, 342,1241947: DOI:10.1126/science.1241974 [PMCID In Progress; NIHMSID#: 544230].